Application | Comment | Organism |
---|---|---|
synthesis | 510 mg of histidine-tagged mouse ubiquitin-activating enzyme E1 can be easily obtained from a 1 l Escherichia coli culture. A low temperature during the protein induction step is critical to obtain an active enzyme | Mus musculus |
Cloned (Comment) | Organism |
---|---|
expresion in Escherichia coli | Mus musculus |
expressed in Escherichia coli BL21(DE3) using pET28-mE1 plasmid | Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Mus musculus | 5829 | - |
soluble | - |
Mus musculus | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein, HIS-Select nickel affinity chromatography, further steps, ion exchange chromatography, followed by gel filtration | Mus musculus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + ubiquitin + [E1 ubiquitin-activating enzyme]-L-cysteine = AMP + diphosphate + S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine | in the ATP-dependent activation reaction, ubiquitin is covalently attached to a cysteine residue of the E1 component through a thiol ester bond | Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Mus musculus | - |
Storage Stability | Organism |
---|---|
-70°C, recombinant enzyme, no detectable loss of activity | Mus musculus |
-70°C, the storage of the recombinant mouse E1 does not result in a detectable denaturation of the enzyme | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + ubiquitin + [ubiquitin-activating protein E1]-L-cysteine | - |
Mus musculus | AMP + diphosphate + [ubiquitin-activating protein E1]-S-ubiquitinyl-L-cysteine | - |
? | |
additional information | E1 activity is assesssed by the capacity of the enzyme to form a thiol ester conjugate with ubiquitin in an ATP-dependent process and to transfer this activated ubiquitin molecule to an conjugating enzyme | Mus musculus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ubiquitin-activating enzyme (E1) | - |
Mus musculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Mus musculus |